Fig 2: Immunostaining of TNF in various brain regions of sham and endometriotic mice. A Representative immunohistochemistry images of TNF in sham controls and endometriotic mice on day 16 and B day 32, the black bar in the lower right image is equal to 150 and 50 µm for the images and the insets, respectively. C–F No difference in TNF expression was observed in the cortex, hippocampus, thalamus, and hypothalamus on days 16 and 32. Values represent mean ± standard error mean (SEM), n = 5–6 mice/timepoint

Fig 3: Suppressing of PDK1 accelerated synovium inflammation. A Representative immunohistochemistry images of F4/80 staining for mice at sham,4 or 8 weeks after DMM with or without jx06. B Representative immunohistochemistry images of TNF-a staining for mice at sham,4 or 8 weeks after DMM with or without jx06. C Quantification of absolute number of F4/80.+ cells in synovium in the knee at sham or 4 weeks post-surgery. D The mean ratio of integrated optical density (IOD) to area (IOD/area) was used to semi-quantify TNF-a amount. E Model of PDK1 inhibitor modulates ECM degradation and synovium inflammation in mechanical stress-induced OA. Horizontal lines and error bars show the mean ± SD (n = 3 mice per group). * = P < 0.05; ** = P < 0.01; *** = P < 0.001